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What is Phloxine B (Acid Red 92)?
Phloxine B, often known as phloxine, is a red, water-soluble dye. It is produced from fluorescein, but it is distinct in that it has four bromine atoms in positions 2, 4, 5, and 7 of the xanthene ring and four chlorine atoms in the carboxyphenyl ring. It has an absorption maximum around 540 nm and an emission maximum around 564 nm.
Macsen Laboratories is the leading manufacturer and supplier of Phloxine B (Acid Red 92) with ISO certification.
| PRODUCT SPECIFICATIONS | |
|---|---|
| Name of Product | Phloxine B (Acid Red 92) |
| IUPAC Name | disodium;2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-3-oxospiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate |
| Synonyms | Cyanosin; Cyanosine; Eosine bluish; Eosine Blue; Cyanosin B; Eosin Blue; Phloxine P; Phloxin B; Eosine I Bluish; Acid red 92; C.I. 45410; D & C Red no. 28 |
| CAS No | 18472-87-2 |
| Molecular Formula | C20H2Br4Cl4Na2O5 |
| Molecular Weight | 829.6 g/mol |
| Pubchem CID | 6433718 |
TECHNICAL SPECIFICATIONS
| SR. No | Criteria | Limit/Specification |
|---|---|---|
| 1 | Appearance (Color) | Dark Red |
| 2 | Appearance (Form) | Powder |
| 3 | Solubility (Color) | Red |
| 4 | Solubility (Turbidity) | Conforms to requirements C=1mg/ml; Water |
| 5 | Infrared spectrum | Conforms to Structure |
| 6 | Absorptivity (A1%, 1CM In 50% Ethanol At 548) | 1150- 1300 |
| 7 | Lambda Max | 546 -550 nm |
Uses
- Phloxine B is used for coloring drugs and cosmetics in the United States and is used as colorant for candy, cakes, sausages, confectionery products and biscuits in Japan. The dosage is 5~100mg/kg.
- It serves as an adsorption indicator for Mallory’s brilliant pink-methylene blue staining.
- It also functions as a  biological stain and a viability dye.
Resources
Biological Stains | Classification, Examples & Uses
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