Bromophenol Blue is an organobromide compound and a member of phenols. It is a pH indicator dye commonly used in laboratory settings. It changes color depending on the acidity or alkalinity of a solution, shifting from yellow (acidic) to blue (alkaline) as the pH increases. It’s often employed in various chemical and biological experiments to monitor pH changes. It shows maximum absorbance at 592 nm.
|Name of Product||Bromophenol Blue|
|Synonyms||Albutest; Tetrabromphenol Blue; 3,3',5,5'-Tetrabromophenolsulfonphthalein; 3′,3″,5′,5″-tetrabromophenolsulfonphthalein; BPB; Bromphenol Blue Sultone Form|
|Molecular Weight||670.0 g/mol|
|1||Appearance (form)||Crystalline Solid Powder|
|2||Appearance (color)||Dark Bluish-Black|
|4||Solubility|| Freely soluble in NaOH solutions
Soluble in Methanol, Ethanol and Benzene
Soluble in Water (3 mg/mL)
Bromophenol Blue works as a pH indicator by changing color in response to pH variations. It’s typically yellow in acidic conditions and transitions to blue as the solution becomes more alkaline. This color shift occurs due to changes in the electronic structure of the molecule as it donates or accepts protons (H⁺ ions), making it a useful tool for tracking pH changes in various laboratory applications.
Some of the common uses of Bromophenol Blue are as follows-
Q. How to make Bromophenol Blue indicator solution?
Bromophenol Blue indicator solution can be prepared by dissolving 0.1 g of Bromophenol Blue with gentle heating in 1.5 ml of 0.1 M NaOH and 20 ml of ethanol (95%) and add sufficient water to produce 100 ml.
Q. Is Bromophenol Blue toxic?
Bromophenol Blue is considered mildly risky when it comes to skin contact (causing irritation), eye contact (causing irritation), ingestion, or inhalation.
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