Auramine O is an aniline dye and is obtained by combining 4,4′-carbonimidoylbis(N,N-dimethylaniline) with one molar equivalent of hydrogen chloride. It is a diarylmethane dye and functions as both a fluorochrome and a histology stain.
|Name of Product||Auramine O|
|Synonyms||Auramine hydrochloride; Basic yellow 2; Pyocatanium aureum; Aizen auramine; Pyoktanin yellow; Canary yellow, pyoktanin; Auramin; Benzophenoneidum; Calcozine Yellow OX|
|Molecular Weight||303.8 g/mol|
|Color Index No||41000|
|1||Appearance (form)||Solid Powder or Flakes|
|3||Melting Point||>250 °C (dec)|
|4||Solubility||10 mg/ml in Water
60 mg/ml in Ethylene glycol methyl ether
20 mg/ml in Ethanol
|5||Solubility (Color)||Clear to hazy, Yellow to very deep yellow|
Q. What fluorescence is emitted by Auramine O?
When exposed to blue light (450–500 nm), Auramine O becomes excited and emits light in the range of around 500 nm to 700 nm (green–yellow–red).
Q. What does Auramine O stain?
Auramine O stain is utilized for the direct detection of mycobacteria in clinical specimens. It binds to mycolic acids and emits light when exposed to ultraviolet light. Acid-fast organisms (mycobacteria) thus appear yellow or orange in the UV light.