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What is Basic Fuchsin (Fuchsine)
Basic fuchsin also known as Fuchsine or Rosaniline hydrochloride is a fluorescent triphenylmethane dye composed of rosaniline, magenta II, pararosaniline and new fuchsine. It is an amine salt and has three amine groups, two primary amines and a secondary amine. It occurs as a dark green powder but becomes magenta when dissolved in water.
The fuchsin dye is the most commonly employed in staining operations. It’s utilised in Gomori’s aldehyde-fuchsin method, periodic acid Schiff method, and Endo media to distinguish E.coli and K.aerogenes bacteria. Basic fuchsin is also employed as a counter-stain in Gram’s staining.
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| PRODUCT SPECIFICATIONS | |
|---|---|
| PRODUCT NAME | Basic Fuchsin |
| IUPAC Name | 4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydrochloride |
| SYNONYMS | Rosaniline hydrochloride; Basic violet 14; Fuchsin basic; Fuchsin; Rosaniline; Basic Magenta; Diamond Fuchsin; Fuchsine |
| CAS No | 632-99-5 |
| Molecular Formula | C20H20ClN3 |
| Molecular Weight | 337.8 g/mol |
| Pubchem CID | 12447 |
| Pubchem SID | 462770365 |
TECHNICAL SPECIFICATIONS
| SR. No | Criteria | Limit/Specification |
|---|---|---|
| 1 | Appearance | Dark green Crystals or powder or crystalline powder |
| 2 | Loss on Drying: (at 105°C) | ≤15% |
| 3 | Infrared spectrum | Conforms to Structure |
| 4 | Lambda-max (UV-Vis) | 547-557 nm |
| 5 | Solubility (Color) | Red |
| 6 | Specific Absorbance (E 1%/1cm) | ≥1500 at lambda max (50% aq. ethanol) |
Uses
- Basic Fuchsin is mainly used as a dye or dye intermediates in colouring textile fibres, fabrics, paper products, cotton and wool.
- In solution with phenol, also called Carbolic acid, it is used as an accentuator in staining thus facilitating the staining process.
- It is widely used in biology to stain the nucleus of cells and is also a component of Lactofuchsin, used for Lactofuchsin mounting.
- It is also used for the Ziehl–Neelsen and another similar acid-fast staining of the mycobacteria which cause tuberculosis, leprosy etc.
FAQs
Q. What is the color of Basic Fuchsin?
Basic Fuchsin is a dark green powder that, when dissolved in water, turns magenta in color.
Q. What is the difference between Acid fuchsin and Basic fuchsin?
Both are different chemical dyes. In fact, Acid fuchsin is the sodium sulfonate derivative of basic fuchsin.
Q. What does Basic Fuchsin stain?
It stains mucopolysaccharides and glycoproteins.
Q. Is Basic fuchsin the same as carbol Fuchsin?
Carbol Fuchsin is a mixture of phenol and Basic fuchsin.
Resources
Biological Stains | Classification, Examples & Uses
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