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What is Leishman Stain?
Leishman stain, also known as Leishman’s stain, is a type of the Romanowsky stain, and is similar to Wright Stain. It is also known as eosin-polychrome methylene blue as it is composed of a methanolic mixture of “polychromed” methylene blue (i.e., demethylated into several azure colors) and eosin. The Leishman stain was developed by Scottish pathologist William Boog Leishman.
| PRODUCT SPECIFICATIONS | |
|---|---|
| PRODUCT NAME | Leishman Stain |
| SYNONYMS | Hematology Stain, Eosin-polychrome methylene blue |
| CAS No | 12627-53-1 |
| Molecular Formula | C6H5NO5 |
| Molecular Weight | 171.038 g/mol |
TECHNICAL SPECIFICATIONS
| SR. No | Criteria | Limit/Specification |
|---|---|---|
| 1 | Appearance | Dark Green crystalline powder |
| 2 | Solubility | 0.1% Methanolic soln. is clear blue coloured with greenish fluorescence. |
| 3 | Loss on drying | Max. 8.0% |
| 4 | Absorptivity of 0.1 % soln. inMethanol at 525 nm | NLT 650 |
| 5 | Absorptivity of 0.1 % soln. in Methanol at 641 nm | NLT 900 |
Uses
- Leishman Stain is utilized in the field of microscopy for the purpose of staining blood smears to differentiate between and identify white blood cells, malaria parasites, and trypanosomas.
- In the field of hematology, it is used to separate and identify distinct types of leukocytes (white blood cells).
- It functions similarly to fluorescent stains for malaria parasite identification.
- It is also used for bone marrow staining.
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