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Leucocrystal Violet (603-48-5)

Leucocrystal Violet Chemical Structure

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What is Leucocrystal Violet?

Leucocrystal Violet (LCV) is a cationic triarylmethane dye and a benzenoid aromatic compound. It is a colorless dye and is a reduced version of gentian violet (crystal violet). It has a high affinity to cellulosic materials as well as protein-based substances and is therefore used in the detection of antimony in environmental and biological samples using spectrophotometric techniques.

Macsen Laboratories is the leading manufacturer and supplier of Leucocrystal Violet with ISO certification.
PRODUCT NAME Leucocrystal Violet
IUPAC Name 4-[bis[4-(dimethylamino)phenyl]methyl]-N,N-dimethylaniline
SYNONYMS Leucocrystal Violet; Leucogentian Violet; C.I. Basic Violet 3; Leucomethyl green; Crystal Violet leucobase; Tris(4-dimethylaminophenyl)methane
CAS No 603-48-5
Molecular Formula C25H31N3
Molecular weight 373.5 g/mol
PubChem CID 69048


SR. No Criteria Limit/Specification
1 Appearance Grayish to white lavender powder
2 Infrared spectrum Conforms to Structure
3 Wavelength (C = 0.01 G/L IN 0.1N HCL IN MEOH)
Extinction Coefficient
257 - 263 nm
≥ 700
4 Wavelength
Extinction Coefficient
201 - 207 nm
≥ 38000
5 Wavelength
587 - 593 nm
≤ 0.01

77.6 - 83.2 %
10.8 - 11.6 %


  • The hemoglobin in the blood reacts with Leucocrystal Violet and hydrogen peroxide, turning the blood impression purple/violet. As a result, the dye is frequently used to stain blood remains on porous and non-porous surfaces. However, because this reagent also includes hydrogen peroxide, it may produce foaming and loss of detail when used on strong bloodstain.
  • Histamine-producing lactobacilli in cheese have been identified using LCV.

Biological Stains | Classification, Examples & Uses

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