Leucocrystal Violet

Leucocrystal Violet Chemical Structure

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PRODUCT NAME Leucocrystal Violet
IUPAC Name 4-[bis[4-(dimethylamino)phenyl]methyl]-N,N-dimethylaniline
SYNONYMS Leucocrystal Violet; Leucogentian Violet; C.I. Basic Violet 3; Leucomethyl green; Crystal Violet leucobase; Tris(4-dimethylaminophenyl)methane
CAS No 603-48-5
Molecular Formula C25H31N3
Molecular weight 373.5 g/mol
PubChem CID 69048


SR. No Criteria Limit/Specification
1 Appearance Grayish to white lavender powder
2 Infrared spectrum Conforms to Structure
3 Wavelength (C = 0.01 G/L IN 0.1N HCL IN MEOH)
Extinction Coefficient
257 - 263 nm
≥ 700
4 Wavelength
Extinction Coefficient
201 - 207 nm
≥ 38000
5 Wavelength
587 - 593 nm
≤ 0.01

77.6 - 83.2 %
10.8 - 11.6 %


Cationic triarylmethane dye Leucocrystal Violet (LCV) is colourless because it is a reduced version of gentian violet (crystal violet). It prefers cellulose as well as proteinaceous substrates. The haemoglobin in the blood reacts with LCV and hydrogen peroxide, turning the blood impression purple/violet. As a result, the dye is frequently used to stain blood remains on porous and non-porous surfaces. Histamine-producing lactobacilli in cheese have been identified using LCV.

The heme-group in blood interacts with LCV to produce a violet hue. To detect and enhance or provide contrast to bloodstains patterns its application is used. Because this reagent includes hydrogen peroxide, it may produce foaming and loss of detail when used on strong bloodstains. On porous surfaces, LCV is commonly employed.
Because LCV interacts with heme, it can be used as a blood test. The Laboratory, on the other hand, does not employ this as a presumptive test for blood.

Caution: Colour changes may result from photo-ionization.

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