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Lissamine Green (11096-63-2)

Lissamine Green Chemical Structure

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What is Lissamine Green

Lissamine Green (LG)  is a synthetically produced organic acid dye with two aminophenyl groups. It has broad applicability as a dye and is widely used in cosmetics and food additives. It is also available for ophthalmic use as individually packaged, sterile strips.

It is used as a stain to diagnose ocular surface disease. Ocular surface epithelial cells without mucin or glycocalyx protection, as well as injured cells, are stained by Lissamine Green. It is also the preferred dye for staining the bulbar conjunctiva.

Macsen Laboratories is the leading manufacturer & supplier of Lissamine Green dye with ISO certification.
Name of Product Lissamine Green
IUPAC Name 3-[[4-[(2-chlorophenyl)-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]methyl]-N-ethylanilino]methyl]benzenesulfonate
Synonyms Erio green; Lissamina Verde; verde lisamina; Verde di lissamina; Vert Lissamine; Acid green S; Wool green S or C; Fast light green; vert de lissamine
CAS No 11096-63-2
Molecular Formula C37H34ClN2O6S2-
Molecular Weight 702.3 g/mol
Pubchem CID 5182671

Chemical Properties

SR. No Criteria Limit/Specification
1 Appearance Dark Green Powder
2 Total Dye content >80%
3 Loss on Drying (at 135°C) <20%
4 Water Insoluble matter
(percent by mass)
5 Combined Ether extracts
(percent by mass)
6 Subsidiary dyes
(percent by mass)
7 Dye Intermediates
(percent by mass)


Side effects

  • Short-term blurring
  • Transient stinging
  • Staining of the eyelids in the case of overspill tearing following application

No other general side effects are reported from the dye.


Q. What is the difference between Rose Bengal and Lissamine Green?

According to studies, rose bengal and lissamine green have comparable staining characteristics, however, lissamine green is much well-tolerated by patients.

Q. What is the difference between Fluorescein and Lissamine green?

  • Fluorescein stains between the cells whereas Lissamine Green stains the cells themselves.
  • Fluorescein gives clearer staining of the cornea and Lissamine Green appears clearly, particularly on the conjunctiva.

Q. Does lissamine green stain cornea?

Lissamine Green stains the conjunctiva and not the cornea.

Q. What are Lissamine Green Strips?

Lissamine Green Strips are specifically manufactured sterile ophthalmic strips used for the diagnosis of dry eye condition and the identification of corneal cells that are lacking or damaged.


Biological Stains | Classification, Examples & Uses

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