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Acid Red 94/ Rose Bengal (632-69-9)

Rose Bengal Chemical Structure

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What is Acid Red 94/Rose Bengal

Acid Red 94 or Rose Bengal is an organic compound and belongs to the class of xanthenes. In 1882, Swiss chemist Robert Ghnem synthesized rose bengal as a  4,5,6,7-tetrachloro 2′,4′,5′,7′-tetraiodofluorescein analogue. It is a commonly used dye for detecting damage to the ocular surface epithelium in ocular surface diseases like dry eye and herpetic keratitis.

Macsen Laboratories is the leading manufacturer & supplier of Rose Bengal with ISO certification.
Name of Product Rose bengal disodium salt
IUPAC Name disodium;4,5,6,7-tetrachloro-2',4',5',7'-tetraiodo-3-oxospiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate
Synonyms Rose Bengal sodium salt; Xantryl; Food Red No. 105, sodium salt; PV 10; C.I. Acid Red 94; Rosa Bengala; Rosa del Bengala; Rose Bengale
CAS No 632-69-9
Molecular Formula C20H2Cl4I4Na2O5
Molecular Weight 1017.6 g/mol
Other available variants Rose Bengal Sodium (11121-48-5)
Rose Bengal Lactone (4159-77-7)
Pubchem CID 69438
Pubchem SID 469688486

Chemical Properties

SR. No Criteria Limit/Specification
1 Appearance (Form) Solid powder
2 Appearance (Color) Dark Violet
3 λmax 549 nm
4 Storage temp. Room temp.


  • It has been used as a diagnostic agent for more than 50 years to identify liver and eye cancer.
  • Its sodium salt is frequently employed in eye drops to color-code injured conjunctival and corneal cells, helping to identify eye injury.
  • Clinical trials for melanoma, breast cancer, and neuroendocrine tumours are currently being conducted with PV-10 (an injectable version of rose bengal).
  • Additionally, studies on rose bengal have revealed that it can treat colon cancer and stop the growth and spread of ovarian cancer.
  • It is being investigated as a potential tool for making nano sutures.
  • Further, the stain is used to prepare foraminifera for microscopic examination, enabling the differentiation between forms that were alive or dead at the time of collecting.


Q. What is Rose Bengal test?

The Rose Bengal test (RBT) is a rapid slide-type agglutination assay performed with a stained B. abortus suspension at pH 3.6–3.7 for the serological diagnosis of brucellosis.

Q. What is the difference between Rose Bengal stain and fluorescein?

Rose Bengal is actually a derivative of fluorescein, however, unlike fluorescein it is not a vital dye. Fluorescein staining manifests whenever there is disruption of cell-cell junctions whereas rose bengal staining ensues whenever there is deficiency of preocular tear film protection. Fluorescein is distinguishable from rose bengal by its absence of inherent toxicity and photodynamic activity.


Biological Stains | Classification, Examples & Uses

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