Safranin Chemical Structure

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What is Safranin?

Safranin, also known as Safranin O or Basic Red 2, is a lipophilic cationic dye. It is a biological dye that is frequently utilized as a counter-stain in a variety of staining methods, including gram staining.

Macsen Laboratories is the leading manufacturer and supplier of Safranin dye with ISO certification.
Name of Product Safranin
IUPAC Name 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine;chloride
Synonyms Basic Red 2; Safranine O; Safranin T; Gossypimine; Safranin O; Safranine T
CAS No 477-73-6
Molecular Formula C20H19ClN4
Molecular Weight 350.8 g/mol
Pubchem CID 2723800


SR. No Criteria Limit/Specification
1 Appearance (form) Solid powder
2 Appearance (color) Brownish-Red to Dark Brown
3 Odor No characteristic odor
4 Solubility Soluble in water and ethanol
5 Melting Point > 240°C
6 Loss on Drying (at 110°C) NMT 15.0 %



Q. What does Safranin do in gram staining?

Safranin is used as a counterstain in gram staining. It stains the gram-negative cells pink or red, thus facilitating the classification of bacterial species into gram-positive bacteria and gram-negative bacteria.

Q. Is Safranin acidic or basic?

Safranin is a basic biological dye.

Q. How does Safranin affect gram positive cells?

Safranin is not able to stain gram-positive bacteria due to the retention of primary stain in their thick layered peptidoglycan cell wall.

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