Safranin, also known as Safranin O or Basic Red 2, is a lipophilic cationic dye. It is a biological dye that is frequently utilized as a counter-stain in a variety of staining methods, including gram staining.
|Name of Product||Safranin|
|Synonyms||Basic Red 2; Safranine O; Safranin T; Gossypimine; Safranin O; Safranine T|
|Molecular Weight||350.8 g/mol|
|1||Appearance (form)||Solid powder|
|2||Appearance (color)||Brownish-Red to Dark Brown|
|3||Odor||No characteristic odor|
|4||Solubility||Soluble in water and ethanol|
|5||Melting Point||> 240°C|
|6||Loss on Drying (at 110°C)||NMT 15.0 %|
Q. What does Safranin do in gram staining?
Safranin is used as a counterstain in gram staining. It stains the gram-negative cells pink or red, thus facilitating the classification of bacterial species into gram-positive bacteria and gram-negative bacteria.
Q. Is Safranin acidic or basic?
Safranin is a basic biological dye.
Q. How does Safranin affect gram positive cells?
Safranin is not able to stain gram-positive bacteria due to the retention of primary stain in their thick layered peptidoglycan cell wall.
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