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Safranin, also known as Safranin O or Basic Red 2, is a lipophilic cationic dye. It is a biological dye that is frequently utilized as a counter-stain in a variety of staining methods, including gram staining.
PRODUCT SPECIFICATIONS | |
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Name of Product | Safranin |
IUPAC Name | 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine;chloride |
Synonyms | Basic Red 2; Safranine O; Safranin T; Gossypimine; Safranin O; Safranine T |
CAS No | 477-73-6 |
Molecular Formula | C20H19ClN4 |
Molecular Weight | 350.8 g/mol |
Pubchem CID | 2723800 |
SR. No | Criteria | Limit/Specification |
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1 | Appearance (form) | Solid powder |
2 | Appearance (color) | Brownish-Red to Dark Brown |
3 | Odor | No characteristic odor |
4 | Solubility | Soluble in water and ethanol |
5 | Melting Point | > 240°C |
6 | Loss on Drying (at 110°C) | NMT 15.0 % |
Q. What does Safranin do in gram staining?
Safranin is used as a counterstain in gram staining. It stains the gram-negative cells pink or red, thus facilitating the classification of bacterial species into gram-positive bacteria and gram-negative bacteria.
Q. Is Safranin acidic or basic?
Safranin is a basic biological dye.
Q. How does Safranin affect gram positive cells?
Safranin is not able to stain gram-positive bacteria due to the retention of primary stain in their thick layered peptidoglycan cell wall.
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