Wright's Stain Chemical Structure

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What is Wright Stain?

Wright stain is a polychromatic stain made up of Eosin and Methylene Blue. It is a modified Romanowsky stain and is named for James Homer Wright who devised the stain in 1902. It is a histological stain that assists in the classification of the various types of blood cells.

Macsen Laboratories is the leading manufacturer and supplier of Wright Stain with ISO certification.
Name of Product Wright Stain
IUPAC Name [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;2-(2,4,5,7-tetrabromo-3,6-dihydroxyxanthen-10-ium-9-yl)benzoic acid
Synonyms Eosin-Methylene blue; Tache de Wright; Mancha de Wright; Macchia di Wright; Tincion de Wright; Switzerland pigment
CAS No 68988-92-1
Molecular Formula C36H27Br4N3O5S+2
Molecular Weight 933.3 g/mol
Pubchem CID 25113599


SR. No Criteria Limit/Specification
1 Appearance Dark Green Powder
2 Infrared spectrum Conforms to Structure
3 Solubility (Turbidity) Turbid
4 Solubility (Colour) Dark Blue to Very Dark Blue
5 A 1% methanol@524nm Min. 650
6 Loss on drying Max. 8%
7 Assay (HPLC) ≥ 4 %


  • The Wright Stain is an agent that is frequently utilized in hematology laboratories for the purpose of performing regular staining procedures on peripheral blood smears.
  • It is also used for staining bone marrow aspirates and urine samples to identify Urinary tract Infection (UTI).
  • In the field of cytogenetics, Wright Stain is used for the purpose of staining chromosomes in order to make the diagnosis of syndromes and disorders easier.
  • The Wright stain distinguishes easily between the blood cells and is commonly used for doing differential white blood cell counts, which are routinely performed whenever diseases such as infection or leukemia are suspected.


Q. What is the principle of Wright’s stain?

Hemoglobin, eosinophilic granules, and cytoplasm will be stained from orange to pink by the acidic dye (eosin) of the Wright Stain. While the basic dye (methylene blue) of the Wright Stain assists in staining the acidic components (such as the nucleus containing nucleic acid) in different shades of blue. Both components of the dye stain the neutral components of the cell, resulting in a range of colors.

Q. What is the difference between Wright Stain and Giemsa Stain?

  • The primary distinction between Giemsa stain and Wright stain is that Giemsa stain is used to identify chromosomal abnormalities by staining chromosomes. However, Wright stain is utilized to distinguish between different types of blood cell.
  • Moreover, Giemsa stain is composed of Azure B, Methylene blue, and Eosin dye, whereas Wright stain is composed of Eosin and Methylene blue dye.

Biological Stains | Classification, Examples & Uses

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