Wright stain is a polychromatic stain made up of Eosin and Methylene Blue. It is a modified Romanowsky stain and is named for James Homer Wright who devised the stain in 1902. It is a histological stain that assists in the classification of the various types of blood cells.
|Name of Product||Wright Stain|
|IUPAC Name||[7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;2-(2,4,5,7-tetrabromo-3,6-dihydroxyxanthen-10-ium-9-yl)benzoic acid|
|Synonyms||Eosin-Methylene blue; Tache de Wright; Mancha de Wright; Macchia di Wright; Tincion de Wright; Switzerland pigment|
|Molecular Weight||933.3 g/mol|
|1||Appearance||Dark Green Powder|
|2||Infrared spectrum||Conforms to Structure|
|4||Solubility (Colour)||Dark Blue to Very Dark Blue|
|5||A 1% methanol@524nm||Min. 650|
|6||Loss on drying||Max. 8%|
|7||Assay (HPLC)||≥ 4 %|
Q. What is the principle of Wright’s stain?
Hemoglobin, eosinophilic granules, and cytoplasm will be stained from orange to pink by the acidic dye (eosin) of the Wright Stain. While the basic dye (methylene blue) of the Wright Stain assists in staining the acidic components (such as the nucleus containing nucleic acid) in different shades of blue. Both components of the dye stain the neutral components of the cell, resulting in a range of colors.
Q. What is the difference between Wright Stain and Giemsa Stain?