Brilliant Blue G (6104-58-1)

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What is Brilliant Blue G?

Brilliant Blue g is an essential ingredient for many different colorimetric protein gel stains. The suffix “G” in the name is an abbreviation for “green” as the blue colour of the dye has a slight greenish tint.

Mechanism of Action

During ophthalmic procedures, Brilliant blue G (BG) is used in an ophthalmic solution to stain the internal limiting membrane (ILM) of the eye. The internal limiting membrane (ILM) is a thin, transparent barrier that separates the retina from the eye’s vitreous body.  Since this membrane is thin and transparent, it might be difficult to detect during eye surgeries requiring high degrees of visual precision. The BG gives a strong blue color, which facilitates the identification of the ILM. BG stains the eye’s internal limiting membrane (ILM) without coloring the epiretinal membrane or the retina, making surgical removal simpler.

Uses

• Brilliant blue G, also known by its trade names ILM Blue and Brilliant Peel, is a stain that is utilised as an aid for surgeons performing retinal surgery. In the United States, it received approval for use in humans in the month of December 2019 and in Canada in January 2021.
• In 2009, scientific trials were conducted using brilliant blue G to cure spinal injuries in laboratory mice.
• The Bradford assay uses the spectral properties of Coomassie brilliant blue G-250 to estimate the amount of protein in a solution.
• After SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis), brilliant blue g has been used to detect protein bands.
• It is also used in fingerprint analysis.

FAQs

Q. What is Coomassie brilliant blue g-250 ?

Coomassie brilliant blue g 250 is one of the two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry. The “250” originally denoted the purity of the dye. The suffix “G” in the name is an abbreviation for “green” as the blue color of the dye has a slight greenish tint. Imperial Chemical Industries owns the trademark for the term “Coomassie.”

Q. How does Coomassie brilliant blue g-250 bind to a protein ?

The dye bind by means of hydrophobic associations with aromatic residues and electrostatic interactions with protonated basic amino acids (lysine, arginine, and histidine).

Q. Does Coomassie Blue stain all proteins?

It is able to form a non-specific bond with the vast majority of proteins.

Buy the best quality Brilliant Blue-G through Macsen Laboratories.