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Trypan Blue Chemical Structure

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Trypan blue is a dye that may be used as a staining agent to tell the difference between living and dead cells. It’s an essential stain that isn’t absorbed by healthy live cells, but it does stain cells with a broken cell membrane. Only dead cells may be counted this way. The dye exclusion method is another term for the procedure.

Application:- Use in cytotoxicity tests to identify dead and dying cells, as well as for routine cell viability and proliferation experiments.

Trypan Blue Solution, 0.4%, is commonly used as a cell stain in the dye exclusion test to determine cell viability. This test is commonly done while counting cells with a hemocytometer during normal subculturing, but it can be done at any time when cell viability must be verified fast and correctly. The dye exclusion test is based on the idea that healthy cells do not absorb impermeable colours (such as Trypan Blue), but dead cells do.

US FDA, EUGMP - WC, WHO GMP, ISO, Certified Manufacturer of high quality Trypan blue.
Macsen Laboratories, world’s leading manufacturer and supplier of Trypan blue.
Name of Product Trypan blue
Pharmacopoeial Name Trypan blue
Synonyms Direct Blue 14, diamine blue. Niagara blue, TRYPAN BLUE, VisionBlue, C.I. Direct Blue 14
CAS No 72-57-1
Molecular Formula C34H24N6Na4O14S4
Molar Mass 872.88 g/mol
Pubchem CID 6296
Pubchem SID 462768917
WHO-GMP Available soon
WC Available soon
DMF Available soon


SR. No Criteria Limit/Specification
1 Appearance Solid
2 Solubility in water <0.1 mg/mL in water
3 Absorption Max 608-610 nm
4 Absorptivity (A1%/1cm.) > 600
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