Trypan blue is a dye that may be used as a staining agent to tell the difference between living and dead cells. It’s an essential stain that isn’t absorbed by healthy live cells, but it does stain cells with a broken cell membrane. Only dead cells may be counted this way. The dye exclusion method is another term for the procedure.
Application:- Use in cytotoxicity tests to identify dead and dying cells, as well as for routine cell viability and proliferation experiments.
Trypan Blue Solution, 0.4%, is commonly used as a cell stain in the dye exclusion test to determine cell viability. This test is commonly done while counting cells with a hemocytometer during normal subculturing, but it can be done at any time when cell viability must be verified fast and correctly. The dye exclusion test is based on the idea that healthy cells do not absorb impermeable colours (such as Trypan Blue), but dead cells do.
|Name of Product||Trypan blue|
|Pharmacopoeial Name||Trypan blue|
|Synonyms||Direct Blue 14, diamine blue. Niagara blue, TRYPAN BLUE, VisionBlue, C.I. Direct Blue 14|
|Molar Mass||872.88 g/mol|
|2||Solubility in water||<0.1 mg/mL in water|
|3||Absorption Max||608-610 nm|
|4||Absorptivity (A1%/1cm.)||> 600|